rev: July 27, 2002
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(for in vitro research use only)
RDI Divison of Fitzgerald Industries Intl offers a wide line of antigens for use in histochemistry, ELISA, and further manufacturing use. Please inquire for other antigens or antibodies to use with these products. Please inquire for Bulk quantities-significant discounts available!
SAMPLE SPEC SHEET: HUMAN GH (GROWTH HORMONE)
-for in vitro research use only-not for internal or external administration to humans or animals
-hGH (human Growth Hormone) is produced in the anterior pituitary gland and regulates growth and metabolism of most human tissues.Natural growth hormone is 22K dalton and a 20K Dalton variant is present in human pituitary glands.
BATCH SPECIFIC SPEC SHEET: Human Growth Hormone-High Purity
lot# $562.00/1mg $250.00/100ug
Reference Prep: 1st IS 80/505 WHO
Purity: >95% by SDS-Page
Level of Contaminants: hTSH 0.006% 0.0006 IU/mg ACS180
hLH 0.004% 0.4IU/mg Abbott IMX
hFSH 0.005% 0.2IU/mg Abbott IMX
hPRL 0.6% 0.006mg/mg Abbott IMX
Storage: -20 DEG C Upon Receipt
Form: Lyophilized powder, lyophilized from 50mM ammonium bicarbonate
Source: Human Pituitary glands
Reference assay: Human GH Nichols hGH assay
For IN VITRO Research or Further Manufacturing Use Only. NOT FOR IN VIVO USE IN HUMANS OR ANIMALS.
Serum from donors has been tested and found negative for hepatitis BSAg, HCV, HIV1, and HIV2 by currently approved FDA methods. However because no test method can offer complete assurance that HIV, HCV or other infectious agents is absent, this material should be handled at BioSafety Level 2 as recommended for any potentially infectious human serum or blood specimen in the CDC/NIH manula "Biosafety in Microbiological and Biomedical Laboratories," 1984.
FOR IN VITRO or FURTHER MANUFACTURING USE ONLY-NOT FOR USE IN DIAGNOSTICS.
Price: $440.00/1mg $312.00/mg 10 or more Larger
sizes quoted on request
Concentration: 1 mg lyophilized
Specific Activity: 3 U/mg when compared to WHO reference standard
Packaging: sterile filtered prior to lyophilization. The protein was lyophilized from 5mg/ml stock after extensive dialysis against 1.7mg sodium phosphate buffer (0,1mg sodium phosphate monobasic and 1.6mg sodium phosphate dibasic) Endotoxin <0.1ng/ug (1EU/ug) of rhuGH
Derivation: produced in E Coli as a single, non glycosylated. polypeptide chain containing 192 amino acids and having a predicted molecular mass of 22,260 daltons Purified by a proprietary chromatographic technique. amino acid composition in total agreement with the expected amino acid composition of native human growth hormone
Solubility: very soluble in water and most aqueous buffers. Recommend diluting in sterile DI water followed by addition of suitable carrier to any diluted material as 1-3% BSA/HSA.
Purity: > 99% as determined by RP-HPLC, anion- exchange FPLC and analysis by reducing and non-reducing SDS-PAGE Silver stained. <1% dimer and aggregates as determined by silver stained SDS-PAGE gel analysis.
Protein content: A280 extinction coefficient 0.72 for 0.1% 1mg/ml solution.
Storage: Lyophilized material although stable at room temp for several weeks is best stored desiccated at -20 DEG C . After reconstitution, store at 4-8 DEG C for up to 1 month or make working aliquots and store -20 DEG C. Avoid frequent freeze thaw cycles.
This Product Is For In Vitro Research Use Only-not for use in or on humans
RDI Divison of Fitzgerald Industries Intl
34 Junction Square Drive
Concord MA 01742-3049
phone (978) 371-6446 or (800) 370-2222
fax (978) 371-2266